CELL BIOLOGY AND CELL TECHNOLOGY

This course studies the structure and function of cells, the principles and applications of cell culture, and instrumentation in biology and biotechnology. Topics include tools for cell studies; light microscopy; principles of confocal microscopy and image analysis; cellular membranes; organelles; cellular transport (ions transport and ions channels); protein and RNA transport; membrane potentials; action potentials; cell junctions; extracellular matrix; cell to cell interactions; cell to matrix interactions; techniques in animal cell culture; mammalian cells in culture; primary and continuous cell lines; cell types and cell growth parameters; media formulation, growth factors, and design of serum-free media; culture lab facilities and sterilization; mechanism of cryopreservation; techniques in plant cell culture; root and shoot culture; explant regeneration; protoplasts and cell fusion; and secondary metabolites.